T Cell Receptor Signalling Results in Rapid Tyrosine Phosphorylation of the Linker Protein LAT Present in Detergent-Resistant Membrane Microdomains

Biochem Biophys Res Commun. 1998 Jul 20;248(2):356-60. doi: 10.1006/bbrc.1998.8857.

Abstract

Stimulation of human T cell line Jurkat results in rapid tyrosine phosphorylation of a 35-38 kDa protein that is found in large and buoyant detergent-resistant membrane microdomains containing also glycosylphosphatidylinositol (GPI)-anchored proteins, glycolipids and Src-family protein tyrosine kinases ("GPI-microdomains"). The pp35-38 was found to be identical to LAT, a recently cloned key component of the T-cell receptor signalling pathway. Moreover, a modified form of protein tyrosine kinase Lck (pp60) was newly detected in the GPI-microdomains of the anti-CD3-stimulated Jurkat cells. These data support the idea that GPI-microdomains play important roles in immunoreceptor signalling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Carrier Proteins / physiology*
  • Detergents / pharmacology
  • Glycosylphosphatidylinositols / metabolism
  • Humans
  • Immunoblotting
  • Intracellular Signaling Peptides and Proteins
  • Jurkat Cells
  • Kinetics
  • Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / physiology
  • Membrane Proteins*
  • Phosphoproteins / physiology*
  • Phosphorylation
  • Proteins / analysis
  • Receptors, Antigen, T-Cell / physiology*
  • Signal Transduction / physiology*
  • Tyrosine / metabolism*
  • src-Family Kinases / physiology

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Detergents
  • Glycosylphosphatidylinositols
  • Intracellular Signaling Peptides and Proteins
  • LAT protein, human
  • LNK protein, human
  • Membrane Proteins
  • Phosphoproteins
  • Proteins
  • Receptors, Antigen, T-Cell
  • Tyrosine
  • Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
  • src-Family Kinases