The increase of memory T cell subsets in children with idiopathic nephrotic syndrome

Nephron. 1998;79(3):274-8. doi: 10.1159/000045049.


Two-color and three-color flow cytometry was carried out to determine whether the memory T cells (CD45RO+ T cells) play a major role in lymphocyte dysfunction of 26 children with idiopathic nephrotic syndrome (INS). The INS patients were divided into three groups: (1) 10 patients who were not receiving glucocorticoid hormone (GCH) and were suffering from acute nephrotic state were referred to as N1; (2) 8 patients who were in remission maintained by GCH therapy alone were referred to as N2; (3) 8 patients who were free of GCH therapy for at least 4 months were referred to as N3. Group N1 demonstrated a significant increase in the percentage of CD45RO+CD4+ T cells and CD45RO+CD8+ T cells (p < 0.05) compared with 11 controls, and these subsets were noted to have a tendency to decrease to control levels in groups N2 and N3. Furthermore, interleukin-2 receptor-alpha expressed subsets in CD45RO+CD4+ T cells (CD45RO+CD4+CD25+ T cells) were also increased only in group N1 (p < 0.02). A similar tendency of absolute counts was observed in these subsets. These results suggest that activated memory T cells reflect lymphocyte dysfunction at initial onset or relapse in INS children.

MeSH terms

  • Antibodies, Monoclonal
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • Child
  • Child, Preschool
  • Female
  • Flow Cytometry / methods
  • Humans
  • Image Cytometry / methods
  • Immunologic Memory / immunology*
  • Leukocyte Common Antigens / analysis
  • Lymphocyte Count
  • Male
  • Nephrotic Syndrome / etiology
  • Nephrotic Syndrome / immunology*
  • Receptors, Interleukin-2 / analysis
  • T-Lymphocyte Subsets / chemistry
  • T-Lymphocyte Subsets / cytology*
  • T-Lymphocyte Subsets / immunology


  • Antibodies, Monoclonal
  • Receptors, Interleukin-2
  • Leukocyte Common Antigens