Two novel MYB homologues with changed expression in late embryogenesis-defective Arabidopsis mutants

Plant Mol Biol. 1998 Jul;37(5):819-27. doi: 10.1023/a:1006011002499.

Abstract

Two novel MYB genes (ATMYBR1 and ATMYBR2) were isolated from Arabidopsis thaliana. Binding to a conserved MYB recognition sequence is demonstrated for the ATMYBR1 protein. The expression of both genes is affected by the fus3, lec1 and abi3 mutations causing pleiotropic defects during late embryogenesis and seed maturation including the loss of dormancy and desiccation tolerance. The strong increase of the transcript levels of both MYB genes during very late stages of embryogenesis typically found in wild type is missing in the mutants. Furthermore, the expression of both MYB genes is developmentally regulated in vegetative tissues. The highly conserved repeats (R2 and R3) of the DNA binding MYB domain of both proteins represent chimeric structures combining features typical of plant and animal derived proteins. This demonstrates the existence of a distinct subfamily of animal-like MYB factors in plant genomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / embryology
  • Arabidopsis / genetics*
  • Arabidopsis Proteins
  • Base Sequence
  • Binding Sites
  • Conserved Sequence
  • DNA, Viral / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Enhancer Elements, Genetic / genetics
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant / physiology*
  • Genes, Plant / genetics
  • Genes, Plant / physiology
  • Molecular Sequence Data
  • Mutation
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Polymorphism, Restriction Fragment Length
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-myb
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Seeds / chemistry
  • Sequence Homology, Amino Acid
  • Simian virus 40 / genetics
  • Trans-Activators / genetics

Substances

  • ATR1 protein, Arabidopsis
  • Arabidopsis Proteins
  • CPC protein, Arabidopsis
  • DNA, Viral
  • DNA-Binding Proteins
  • Plant Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myb
  • RNA, Messenger
  • RNA, Plant
  • Trans-Activators

Associated data

  • GENBANK/Z54136
  • GENBANK/Z54137