An as-1-like motif controls the level of expression of the gene for the pathogenesis-related protein 1a from tobacco

Plant Mol Biol. 1998 Jul;37(5):871-83. doi: 10.1023/a:1006003916284.

Abstract

Pathogenesis-related proteins of group 1 (PR-1) are strongly induced in plants by pathogen attack, exposure of the plants to (acetyl)salicylic acid (ASA, SA), and by developmental cues. Functional analysis of the PR-1a promoter identified a region of 139 bp (from -691 to -553) mediating expression of the GUS reporter gene in response to ASA. Inspection of this region revealed two TGACG elements reminiscent of activation sequence-1 (as-1). Recently, as-1 has been reported to be responsive to SA in the context of the CaMV 35S RNA promoter. To address the question of whether the as-1-like sequence may be of functional significance for the expression of the PR-1a gene, gel shift assays were performed with TGA1a, a protein been shown to interact with as-1 in vitro. TGA1a was found to bind to the PR-1a as-1-like sequence with similar specificity and affinity as to as-1. Furthermore, mutations were introduced in the as-1-like sequence in the context of the inducible 906 bp PR-1a promoter which are impaired in binding TGA1a in vitro. Significantly reduced levels of GUS reporter gene activity were obtained with the mutant promoter regions as compared to the wild-type PR-1a promoter in response to all stimuli in transgenic tobacco plants. Yet, mutation of the as-1-like sequence did not abolish induction of reporter gene expression. Taken together, these results suggest that the level of expression of the tobacco PR-1a gene is controlled by an as-1-like sequence motif in the PR-1a upstream region, possibly interacting with a factor related to TGA1a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basic-Leucine Zipper Transcription Factors
  • Caulimovirus / genetics
  • DNA, Plant / metabolism
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Plant / genetics*
  • Genes, Reporter
  • Glucuronidase / genetics
  • Mutation
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plants, Genetically Modified
  • Plants, Toxic*
  • Promoter Regions, Genetic / genetics
  • Recombinant Fusion Proteins
  • Salicylates / pharmacology
  • Salicylic Acid
  • Tobacco / genetics*
  • Transcriptional Activation / genetics*

Substances

  • Basic-Leucine Zipper Transcription Factors
  • DNA, Plant
  • DNA-Binding Proteins
  • Plant Proteins
  • Recombinant Fusion Proteins
  • Salicylates
  • TGA1a protein, Nicotiana tabacum
  • pathogenesis-related proteins, plant
  • Glucuronidase
  • Salicylic Acid