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. 1998 Aug;115(2):287-96.
doi: 10.1016/s0016-5085(98)70195-7.

Metastasis of Human Colon Cancer Is Altered by Modifying Expression of the Beta-Galactoside-Binding Protein Galectin 3

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Metastasis of Human Colon Cancer Is Altered by Modifying Expression of the Beta-Galactoside-Binding Protein Galectin 3

R S Bresalier et al. Gastroenterology. .

Abstract

Background & aims: Galectin 3 is a beta-galactoside-binding protein whose expression has been correlated with advanced tumor stage in the colon, but direct evidence for a role in metastasis is lacking. The current study was designed to more directly establish the role of galectin 3 in colon cancer metastasis.

Methods: Galectin 3 levels were manipulated in human colon cancer cells using eukaryotic expression constructs designed to express the complete galectin 3 complementary DNA in either the sense or antisense orientation. Liver colonization was assessed in athymic mice after splenic-portal inoculation or after spontaneous metastasis during cecal growth.

Results: Introduction of galectin 3 antisense into metastatic colon cancer cells (LSLiM6, HM7) resulted in a significant reduction in galectin 3-specific messenger RNA and total and cell surface galectin 3 protein. Conversely, stable integration of galectin 3 in the sense orientation resulted in an increase in cellular and cell surface galectin 3 in cells of low metastatic potential (LS174T). Reduction in galectin 3 levels was associated with a marked decrease in liver colonization and spontaneous metastasis by LSLiM6 and HM7 cells, whereas up-regulation of galectin 3 resulted in increased metastasis by LS174T cells.

Conclusions: This study provides direct evidence that galectin 3 plays an important role in colon cancer metastasis.

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