The gastric H+,K+-ATPase is a major autoantigen in chronic Helicobacter pylori gastritis with body mucosa atrophy

Gastroenterology. 1998 Aug;115(2):340-7. doi: 10.1016/s0016-5085(98)70200-8.


Background & aims: A subgroup of Helicobacter pylori-infected patients develops autoantibodies to gastric parietal cell canaliculi. The aim of this study was to define the unknown autoantigen.

Methods: We screened 72 H. pylori-infected patients, 5 patients with autoimmune gastritis, and 36 healthy controls for immunoglobulin G autoantibodies to canaliculi by immunohistochemistry. The antigen specificity was determined by immunoprecipitation of the murine gastric H+,K+-adenosine triphosphatase (H+,K+-ATPase) expressed in oocytes and by immunoblotting on human gastric membranes from the body mucosa.

Results: Autoantibodies specific for the conformational peptides of the H+,K+-ATPase were detected in 3% (1/36) of controls, in all patients with autoimmune gastritis (5/5), in 25% (18/72) of H. pylori-infected patients, and in 47% (15/32) of the infected patients with anticanalicular autoantibodies. No other major autoantigen was identified. Atrophy in the gastric body mucosa was found in 60% (9/15) of infected patients with both anticanalicular and anti-H+,K+-ATPase antibodies, but only in 13% (5/37) of infected patients lacking both autoantibodies (P < 0.01).

Conclusions: The gastric H+,K+-ATPase is a major autoantigen in H. pylori-associated antigastric autoimmunity. Thus, anti-H+,K+-ATPase autoantibodies, which are closely linked to classical autoimmune gastritis, are also significant indicators for body mucosa atrophy in chronic H. pylori gastritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Atrophy
  • Autoantibodies / analysis
  • Autoantigens / immunology*
  • Chronic Disease
  • Female
  • Gastric Mucosa / pathology
  • Gastritis / immunology*
  • Gastritis / microbiology*
  • Gastritis / pathology
  • H(+)-K(+)-Exchanging ATPase / immunology*
  • Helicobacter Infections / immunology*
  • Helicobacter pylori*
  • Humans
  • Isoantigens / analysis
  • Lewis Blood Group Antigens / immunology
  • Male
  • Middle Aged
  • Parietal Cells, Gastric / immunology
  • Stomach / enzymology*


  • Autoantibodies
  • Autoantigens
  • Isoantigens
  • Lewis Blood Group Antigens
  • H(+)-K(+)-Exchanging ATPase