The 14-3-3 proteins associate with the plant plasma membrane H(+)-ATPase to generate a fusicoccin binding complex and a fusicoccin responsive system

Plant J. 1998 Mar;13(5):661-71. doi: 10.1046/j.1365-313x.1998.00083.x.


The plasma membrane H(+)-ATPase in higher plants has been implicated in nutrient uptake, phloem loading, elongation growth and establishment of turgor. Although a C-terminal regulatory domain has been identified, little is known about the physiological factors involved in controlling the activity of the enzyme. To identify components which play a role in the regulation of the plant H(+)-ATPase, a fusicoccin responsive yeast expressing Arabidopsis plasma membrane H(+)-ATPase AHA2 was employed. By testing the fusicoccin binding activity of yeast membranes, the C-terminal regulatory domain of AHA2 was found to be part of a functional fusicoccin receptor, a component of which was the 14-3-3 protein. ATP hydrolytic activity of AHA2 expressed in yeast internal membranes was activated by all tested isoforms of the 14-3-3 protein of yeast and Arabidopsis, but only in the presence of fusicoccin, and activation was prevented by a phosphoserine peptide representing a known 14-3-3 protein binding motif in Raf-1. The results demonstrate that the 14-3-3 protein is an activator molecule of the H(+)-ATPase and provides the first evidence of a protein involved in activation of plant plasma membrane H(+)-ATPase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins
  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Arabidopsis / genetics
  • Base Sequence
  • Cell Membrane / enzymology
  • DNA Primers / genetics
  • Enzyme Activation / drug effects
  • Glycosides / metabolism*
  • Glycosides / pharmacology
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism
  • Peptide Fragments / pharmacology
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Protein Binding
  • Proteins / genetics
  • Proteins / metabolism*
  • Proton-Translocating ATPases / genetics
  • Proton-Translocating ATPases / metabolism*
  • Receptors, Cell Surface / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Tyrosine 3-Monooxygenase*


  • 14-3-3 Proteins
  • DNA Primers
  • Glycosides
  • Peptide Fragments
  • Plant Proteins
  • Proteins
  • Receptors, Cell Surface
  • fusicoccin receptor protein, plant
  • fusicoccin
  • Tyrosine 3-Monooxygenase
  • Proton-Translocating ATPases