IL-10 synthesis and secretion by peripheral blood mononuclear cells in haemodialysis patients

Nephrol Dial Transplant. 1998 Jul;13(7):1745-51. doi: 10.1093/ndt/13.7.1745.


Purpose of study: IL-10 may explain the paradox between immunodeficiency and oversecretion of cytokines in chronic haemodialysis (HD) patients. We analysed the secretion of IL-10 by PBMC and the expression of IL-10 mRNA in 10 long-term HD patients (108-276 months), 10 short-term HD patients (3-18 months), and 10 healthy controls.

Results: Spontaneous IL-10 secretion was higher in HD patients than in controls (15 pg/ml vs 2 pg/ml, P = 0.004). It was detected in 13 of 20 patients and in 1 of 10 controls (P = 0.01). IL-10 mRNA expression was also higher in HD patients than in controls. Spontaneous secretions of IL-10 and IL-6 were positively correlated in patients. IL-10 secretion in response to LPS was higher than the upper limit of control range in 4 of 10 long-term HD patients and in no short-term HD patients (P = 0.04). IL-10 mRNA expression was also higher in long-term than in short-term HD patients.

Conclusions: This study demonstrates that IL-10 is spontaneously synthesized and secreted in HD patients, supporting an immunomodulating role in this setting. The greater IL-10-producing capacity in long-term HD patients indicates a chronic effect of haemodialysis on PBMC responsiveness.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Case-Control Studies
  • Female
  • Humans
  • In Vitro Techniques
  • Interleukin-10 / blood*
  • Interleukin-10 / genetics
  • Interleukin-10 / metabolism
  • Interleukin-6 / blood
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology*
  • Lipopolysaccharides / toxicity
  • Male
  • Middle Aged
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Renal Dialysis / adverse effects*
  • Time Factors


  • Interleukin-6
  • Lipopolysaccharides
  • RNA, Messenger
  • Interleukin-10