Kinesin accumulation in chick spinal axonal swellings with beta,beta'-iminodipropionitrile (IDPN) intoxication

Neurosci Lett. 1998 Jun 19;249(2-3):103-6. doi: 10.1016/s0304-3940(98)00400-5.

Abstract

Kinesin is a major molecular motor responsible for anterograde axonal transport. Chicks were injected with beta,beta'-iminodipropionitrile (IDPN) to induce axonal swellings in spinal motor neurons and spinal sensory ganglion neurons. Cylindrical swollen axons were found in the anterior horn and anterior funiculus of the spinal cord, anterior root, and spinal ganglia. All of the axonal swellings were heavily stained with two anti-kinesin monoclonal antibodies. The swellings were mildly stained with an anti-cytoplasmic dynein and anti-tubulin antibodies, and weakly stained with an anti-tau antibody. These suggest the isolated disturbance of kinesin transport with neurofilament accumulation in IDPN intoxication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / metabolism*
  • Axons / pathology
  • Chickens
  • Immunohistochemistry
  • Kinesin / drug effects
  • Kinesin / metabolism*
  • Microtubule Proteins / drug effects
  • Microtubule Proteins / metabolism
  • Motor Neurons / metabolism
  • Motor Neurons / pathology
  • Neurons, Afferent / metabolism
  • Neurons, Afferent / pathology
  • Neurotoxins / pharmacology
  • Nitriles / pharmacology*
  • Spinal Cord Injuries / chemically induced
  • Spinal Cord Injuries / metabolism*

Substances

  • Microtubule Proteins
  • Neurotoxins
  • Nitriles
  • 3,3'-iminodipropionitrile
  • Kinesin