Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins

Dev Genes Evol. 1998 Jul;208(5):290-4. doi: 10.1007/s004270050184.

Abstract

We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1(2)/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / isolation & purification*
  • Drosophila / embryology*
  • Gene Expression Regulation, Developmental / genetics
  • Immunohistochemistry
  • Insect Proteins / immunology*
  • Microscopy, Fluorescence
  • Recombinant Proteins / immunology

Substances

  • Antibodies
  • Insect Proteins
  • Recombinant Proteins