In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting

Nucleic Acids Res. 1998 Aug 15;26(16):3862-4. doi: 10.1093/nar/26.16.3862.

Abstract

The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus nidulans / genetics
  • Aspergillus nidulans / metabolism
  • Aspergillus niger / genetics
  • Aspergillus niger / metabolism
  • Base Sequence
  • Binding Sites / genetics
  • DNA Footprinting / methods*
  • DNA Methylation
  • DNA, Fungal / genetics*
  • DNA, Fungal / isolation & purification
  • DNA, Fungal / metabolism*
  • Fungal Proteins / metabolism*
  • Genes, Fungal
  • Mitosporic Fungi / genetics*
  • Mitosporic Fungi / metabolism*
  • Oligonucleotide Probes / genetics
  • Penicillium chrysogenum / genetics
  • Penicillium chrysogenum / metabolism
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Protein Binding

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Oligonucleotide Probes