Degradation of 1,3-dichloropropene by pseudomonas cichorii 170

Appl Environ Microbiol. 1998 Aug;64(8):2931-6. doi: 10.1128/AEM.64.8.2931-2936.1998.


The gram-negative bacterium Pseudomonas cichorii 170, isolated from soil that was repeatedly treated with the nematocide 1, 3-dichloropropene, could utilize low concentrations of 1, 3-dichloropropene as a sole carbon and energy source. Strain 170 was also able to grow on 3-chloroallyl alcohol, 3-chloroacrylic acid, and several 1-halo-n-alkanes. This organism produced at least three different dehalogenases: a hydrolytic haloalkane dehalogenase specific for haloalkanes and two 3-chloroacrylic acid dehalogenases, one specific for cis-3-chloroacrylic acid and the other specific for trans-3-chloroacrylic acid. The haloalkane dehalogenase and the trans-3-chloroacrylic acid dehalogenase were expressed constitutively, whereas the cis-3-chloroacrylic acid dehalogenase was inducible. The presence of these enzymes indicates that 1, 3-dichloropropene is hydrolyzed to 3-chloroallyl alcohol, which is oxidized in two steps to 3-chloroacrylic acid. The latter compound is then dehalogenated, probably forming malonic acid semialdehyde. The haloalkane dehalogenase gene, which is involved in the conversion of 1,3-dichloropropene to 3-chloroallyl alcohol, was cloned and sequenced, and this gene turned out to be identical to the previously studied dhaA gene of the gram-positive bacterium Rhodococcus rhodochrous NCIMB13064. Mutants resistant to the suicide substrate 1,2-dibromoethane lacked haloalkane dehalogenase activity and therefore could not utilize haloalkanes for growth. PCR analysis showed that these mutants had lost at least part of the dhaA gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkanes / metabolism
  • Allyl Compounds / metabolism*
  • Biodegradation, Environmental
  • Cloning, Molecular
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Bacterial
  • Hydrocarbons, Chlorinated
  • Hydrolases / genetics
  • Hydrolases / isolation & purification
  • Hydrolases / metabolism*
  • Insecticides / metabolism*
  • Mutation
  • Plasmids / genetics
  • Pseudomonas / genetics
  • Pseudomonas / growth & development
  • Pseudomonas / metabolism*
  • Soil Microbiology


  • Alkanes
  • Allyl Compounds
  • Hydrocarbons, Chlorinated
  • Insecticides
  • 1,3-dichloro-1-propene
  • Hydrolases
  • haloalkane dehalogenase