A putative HCO3- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942

FEBS Lett. 1998 Jul 3;430(3):236-40. doi: 10.1016/s0014-5793(98)00662-0.


Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3- uptake in the wild type and IL-2 suggested the presence of two or three HCO3- carriers exhibiting different affinities to HCO3-.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bicarbonates / metabolism*
  • Carbon Dioxide / metabolism
  • Carbonates / metabolism
  • Cloning, Molecular
  • Cyanobacteria / genetics
  • Cyanobacteria / metabolism*
  • DNA Mutational Analysis
  • Gene Library
  • Interleukin-2 / genetics
  • Interleukin-2 / physiology*
  • Kinetics
  • Molecular Sequence Data
  • Mutation
  • Open Reading Frames / genetics
  • Restriction Mapping
  • Sequence Homology, Amino Acid


  • Bicarbonates
  • Carbonates
  • Interleukin-2
  • Carbon Dioxide

Associated data

  • GENBANK/U62616