Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS

FEBS Lett. 1998 Jul 3;430(3):419-23. doi: 10.1016/s0014-5793(98)00706-6.


A quantitative method for detection of amyloid beta peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of A beta 1-40 and A beta 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for A beta 1-40 and A beta 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other A beta peptide species that may also play a role in the onset of AD in humans.

Publication types

  • Comparative Study

MeSH terms

  • Amyloid beta-Peptides / analysis*
  • Animals
  • Brain Chemistry
  • Cell Extracts
  • Chromatography, High Pressure Liquid / methods*
  • Culture Media, Conditioned
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Mass Spectrometry / methods
  • Peptides / analysis*
  • Precipitin Tests / methods*
  • Sensitivity and Specificity
  • Swine


  • Amyloid beta-Peptides
  • Cell Extracts
  • Culture Media, Conditioned
  • Peptides