Cloning and characterization of fiber type-specific ryanodine receptor isoforms in skeletal muscles of fish

Am J Physiol. 1998 Aug;275(2):C401-15. doi: 10.1152/ajpcell.1998.275.2.C401.


We have cloned a group of cDNAs that encodes the skeletal ryanodine receptor isoform (RyR1) of fish from a blue marlin extraocular muscle library. The cDNAs encode a protein of 5,081 amino acids with a calculated molecular mass of 576,302 Da. The deduced amino acid sequence shows strong sequence identity to previously characterized RyR1 isoforms. An RNA probe derived from a clone of the full-length marlin RyR1 isoform hybridizes to RNA preparations from extraocular muscle and slow-twitch skeletal muscle but not to RNA preparations from fast-twitch skeletal or cardiac muscle. We have also isolated a partial RyR clone from marlin and toadfish fast-twitch muscles that shares 80% sequence identity with the corresponding region of the full-length RyR1 isoform, and a RNA probe derived from this clone hybridizes to RNA preparations from fast-twitch muscle but not to slow-twitch muscle preparations. Western blot analysis of slow-twitch muscles in fish indicates the presence of only a single high-molecular-mass RyR protein corresponding to RyR1. [3H]ryanodine binding assays revealed the fish slow-twitch muscle RyR1 had a greater sensitivity for Ca2+ than the fast-twitch muscle RyR1. The results indicate that, in fish muscle, fiber type-specific RyR1 isoforms are expressed and the two proteins are physiologically distinct.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antisense Elements (Genetics)
  • Base Sequence
  • Binding Sites
  • Calcium / pharmacology
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cloning, Molecular
  • Conserved Sequence
  • DNA Primers
  • Fishes
  • Humans
  • Molecular Sequence Data
  • Muscle Fibers, Fast-Twitch / physiology*
  • Muscle Fibers, Slow-Twitch / physiology*
  • Muscle, Skeletal / physiology*
  • Oligonucleotide Probes
  • Phylogeny
  • Rabbits
  • Ranidae
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Restriction Mapping
  • Ryanodine Receptor Calcium Release Channel / biosynthesis
  • Ryanodine Receptor Calcium Release Channel / chemistry
  • Ryanodine Receptor Calcium Release Channel / genetics*
  • Ryanodine Receptor Calcium Release Channel / physiology
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • Antisense Elements (Genetics)
  • DNA Primers
  • Oligonucleotide Probes
  • Recombinant Proteins
  • Ryanodine Receptor Calcium Release Channel
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Calcium