Cloning of the human tissue inhibitor of metalloproteinase-4 gene (TIMP4) and localization of the TIMP4 and Timp4 genes to human chromosome 3p25 and mouse chromosome 6, respectively

Genomics. 1998 Jul 1;51(1):148-51. doi: 10.1006/geno.1998.5362.


We have isolated genomic DNA containing the human tissue inhibitor of metalloproteinases-4 gene (TIMP4) and determined the structure of the exons comprising the gene. Like other members of the TIMP family, the TIMP-4 protein is encoded by five exons. These span 6 kb of genomic DNA, so that TIMP4 is similar in size to Timp1 but considerably smaller than TIMP2 and TIMP3. The exon-intron boundaries of TIMP4 are at locations very similar to those of the other TIMP genes, demonstrating the high degree of conservation of gene structure in this family. The human and mouse TIMP-4 genes map to comparable locations in the respective genomes, localizing to human chromosome 3p25 and mouse chromosome 6.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chromosomes, Human, Pair 3 / genetics*
  • Cloning, Molecular
  • Conserved Sequence
  • Evolution, Molecular
  • Exons
  • Humans
  • In Situ Hybridization, Fluorescence
  • Introns
  • Metalloendopeptidases / antagonists & inhibitors
  • Mice / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Restriction Mapping
  • Tissue Inhibitor of Metalloproteinases / genetics*


  • Tissue Inhibitor of Metalloproteinases
  • tissue inhibitor of metalloproteinase-4
  • Metalloendopeptidases

Associated data

  • GENBANK/AF057528
  • GENBANK/AF057529
  • GENBANK/AF057530
  • GENBANK/AF057531
  • GENBANK/AF057532