Proteins of rat serum: I. Establishing a reference two-dimensional electrophoresis map by immunodetection and microbore high performance liquid chromatography-electrospray mass spectrometry

Electrophoresis. 1998 Jun;19(8-9):1484-92. doi: 10.1002/elps.1150190845.


In the present investigation, we have identified 56 major spots, or spot rows, corresponding to 22 proteins, in the 2-DE pattern of adult male rats. This was done mainly by applying two complementary techniques, namely immunoblotting and high performance liquid chromatography-mass spectrometry (HPLC-MS) peptide mapping. Glycoproteins were characterized by affinity blotting with six lectins. We have also detailed how rat serum differs from human serum in two main respects: (i) relative abundance of individual proteins, which amounts in some cases to a complete absence in either sample, and (ii) varying molecular parameters for homologous proteins. It was thus possible to establish a first-generation reference map of rat serum proteins, which can be accessed through +.html. We hope the present database will be a useful reference for the evaluation of changes in serum protein distribution in the course of pharmacological and toxicological studies. The recognition of species-specific proteins appears of special relevance in this respect.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Blood Proteins / analysis
  • Blood Proteins / standards*
  • Chromatography, High Pressure Liquid*
  • Electrophoresis, Gel, Two-Dimensional*
  • Humans
  • Immunoblotting*
  • Male
  • Mass Spectrometry
  • Mice
  • Peptide Mapping / methods*
  • Rats
  • Rats, Sprague-Dawley
  • Reference Standards


  • Blood Proteins