Dynamics of tandem repetitive Afa-family sequences in Triticeae, wheat-related species

J Mol Evol. 1998 Aug;47(2):183-9. doi: 10.1007/pl00006375.

Abstract

The Afa-family sequences in wheat-related species, Triticeae, are tandem repetitive sequences of 340 bp. All the analyzed Triticeae species carried the sequences in their genomes, though the copy numbers varied about 100-fold among the species. The nucleotide fragments amplified by PCR were cloned and sequenced, and their behavior in the evolution of Triticeae was analyzed by the neighbor-joining (NJ) method. The sequences in genomes with many copies of this family clustered at independent branches of the phylogenic tree, whereas the sequences in genomes with a few copies did not. This may suggest that Afa-family sequences had amplified several times in the evolution of Triticeae, each using a limited number of different master copies. In addition, the sequences of the A and B genomes of hexaploid common wheat indicated that the Afa-family sequences had not evolved in a concerted manner between the genomes. Furthermore, the sequences of each chromosome of the D genome of this species indicated that the sequences had amplified on all over the D-genome chromosomes in a short period.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Edible Grain / classification
  • Edible Grain / genetics*
  • Evolution, Molecular*
  • Gene Amplification
  • Gene Dosage
  • Models, Genetic
  • Molecular Sequence Data
  • Phylogeny
  • Ploidies
  • Poaceae / classification
  • Poaceae / genetics*
  • Repetitive Sequences, Nucleic Acid*
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Triticum / classification
  • Triticum / genetics*

Associated data

  • GENBANK/AB003197
  • GENBANK/AB003198
  • GENBANK/AB003199
  • GENBANK/AB003200
  • GENBANK/AB003201
  • GENBANK/AB003202
  • GENBANK/AB003203
  • GENBANK/AB003204
  • GENBANK/AB003205
  • GENBANK/AB003206
  • GENBANK/AB003207
  • GENBANK/AB003208
  • GENBANK/AB003209
  • GENBANK/AB003210
  • GENBANK/AB003211
  • GENBANK/AB003212
  • GENBANK/AB003213
  • GENBANK/AB003214
  • GENBANK/AB003215
  • GENBANK/AB003216
  • GENBANK/AB003217
  • GENBANK/AB003218
  • GENBANK/AB003219
  • GENBANK/AB003220
  • GENBANK/AB003221
  • GENBANK/AB003222
  • GENBANK/AB003223
  • GENBANK/AB003224
  • GENBANK/AB003225
  • GENBANK/AB003226