Unravelling Golgi Membrane Traffic With Green Fluorescent Protein Chimeras

Trends Cell Biol. 1998 Jan;8(1):16-20. doi: 10.1016/s0962-8924(97)01199-9.

Abstract

An important new tool for investigating how the Golgi receives cargo and maintains its integrity in the face of ongoing secretory traffic has emerged with the advent of green fluorescent protein (GFP) chimeras. GFP chimeras, which can be visualized in the unperturbed environment of a living cell, are being used in a wide variety of applications to study Golgi dynamics. These include time-lapse imaging, double-label and photobleach experiments. These studies are helping to clarify the steps involved in the formation, translocation and fate of transport intermediates associated with the Golgi complex, including the roles of cytoskeletal elements. They are also providing insights into mechanisms of protein retention and localization within Golgi membranes.

Publication types

  • Review

MeSH terms

  • Animals
  • Biological Transport / genetics
  • Golgi Apparatus / genetics
  • Golgi Apparatus / metabolism*
  • Green Fluorescent Proteins
  • Humans
  • Indicators and Reagents
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Recombinant Fusion Proteins / metabolism*

Substances

  • Indicators and Reagents
  • Luminescent Proteins
  • Membrane Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins