GFP illuminates the cytoskeleton

Trends Cell Biol. 1998 Feb;8(2):72-7.


Until recently, cytoskeleton research has relied primarily on immunofluorescence microscopy techniques, requiring fixation and hence killing of the specimen before the analysis. The sole method for visualizing cytoskeletal dynamics in living cells has been the microinjection of purified and fluorescently labelled protein, but technical difficulties have precluded its widespread use. The recent introduction of green fluorescent protein (GFP) has enabled visualization of proteins and cytoskeletal dynamics with only minimal perturbations of the living cell and has opened new horizons for studying the cytoskeleton.

Publication types

  • Review

MeSH terms

  • Animals
  • Cytoskeleton / physiology*
  • Fluorescent Antibody Technique*
  • Green Fluorescent Proteins
  • Indicators and Reagents*
  • Luminescent Proteins*
  • Microscopy, Fluorescence / methods*


  • Indicators and Reagents
  • Luminescent Proteins
  • Green Fluorescent Proteins