Leptomycin B inhibits equine infectious anemia virus Rev and feline immunodeficiency virus rev function but not the function of the hepatitis B virus posttranscriptional regulatory element

J Virol. 1998 Sep;72(9):7593-7. doi: 10.1128/JVI.72.9.7593-7597.1998.

Abstract

Human immunodeficiency virus type 1 Rev export depends upon the presence of the nuclear export signal (NES), a leucine-rich stretch of hydrophobic amino acids. Recently, the nuclear NES-binding receptor has been identified as CRM1 or exportin 1. Rev export has been shown to be CRM1 dependent. The function of the atypical NES-containing Rev-like proteins of equine infectious anemia virus (EIAV) and feline immunodeficiency virus (FIV) is inhibited by leptomycin B, a drug that specifically blocks NES-CRM1 interactions. These data suggest that the function of atypical NES-containing proteins is CRM1 dependent. In contrast to the inhibition of EIAV Rev and FIV Rev, the cytoplasmic accumulation of hepatitis B virus (HBV) posttranscriptional regulatory element (PRE)-containing and Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE)-containing RNAs is not inhibited by leptomycin B treatment. We conclude that the HBV PRE, like the MPMV CTE, functions independently of an NES receptor-exportin 1 interaction.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / antagonists & inhibitors*
  • Cats
  • Fatty Acids, Unsaturated / pharmacology
  • Gene Products, rev / genetics*
  • Hepatitis B virus / genetics*
  • Humans
  • Immunodeficiency Virus, Feline / genetics*
  • Infectious Anemia Virus, Equine / genetics*
  • Karyopherins*
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / antagonists & inhibitors*
  • Protein Sorting Signals*
  • RNA Processing, Post-Transcriptional
  • Receptors, Cytoplasmic and Nuclear*

Substances

  • Carrier Proteins
  • Fatty Acids, Unsaturated
  • Gene Products, rev
  • Karyopherins
  • Nuclear Proteins
  • Protein Sorting Signals
  • Receptors, Cytoplasmic and Nuclear
  • exportin 1 protein
  • leptomycin B