Candidiasis in interferon-gamma knockout (IFN-gamma-/-) mice

J Infect Dis. 1998 Aug;178(2):478-87. doi: 10.1086/515645.

Abstract

Germ-free C57BL/6 x 129 interferon-gamma knockout (IFN-gamma(-/-)) mice and their immunocompetent (+/-, +/+) counterparts were colonized with a pure culture of Candida albicans to assess their natural susceptibility to mucosal and systemic candidiasis of endogenous origin. Colonization with a pure culture of C. albicans was not lethal for adult or neonatal IFN-gamma(-/-) gnotobiotic mice over the 15-week study. The IFN-gamma(-/-) mice were more susceptible to gastric (cardia-antrum section), anorectal, and acute systemic (intravenous challenge) candidiasis than immunocompetent controls, and some IFN-gamma(-/-) mice developed intestinal adenomas after colonization with C. albicans. The enhanced susceptibility of IFN-gamma(-/-) mice, compared with immunocompetent controls, may be associated with a poor proliferative response of spleen cells to C. albicans antigens and a T helper 2 (IgG1) serum antibody response to C. albicans antigens. Thus, IFN-gamma is important for murine resistance to gastric, anorectal, and acute systemic candidiasis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoma / etiology
  • Adenoma / pathology
  • Administration, Oral
  • Animals
  • Antibodies, Fungal / blood
  • Candidiasis / immunology*
  • Cell Division
  • Concanavalin A / pharmacology
  • Disease Models, Animal
  • Disease Susceptibility
  • Female
  • Genotype
  • Germ-Free Life
  • Interferon-gamma / genetics
  • Interferon-gamma / immunology*
  • Intestinal Neoplasms / etiology
  • Intestinal Neoplasms / pathology
  • Lipopolysaccharides / pharmacology
  • Lymphocytes / drug effects
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitogens / pharmacology

Substances

  • Antibodies, Fungal
  • Lipopolysaccharides
  • Mitogens
  • Concanavalin A
  • Interferon-gamma