Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting

Immunity. 1998 Jul;9(1):135-41. doi: 10.1016/s1074-7613(00)80595-6.


Likely creation of mismatches during somatic hypermutation has stimulated interest in the effect of mismatch repair deficiency on the process. Analysis of unselected mutations in the 3' flank of VH rearrangements in germinal center B cells revealed that MSH2 deficiency caused a 5-fold reduced mutation accumulation. This might reflect ectopic effects of the Msh2 disruption; indeed, the mice exhibit other perturbations within the B cell compartment. However, that MSH2 (or factors dependent upon it) plays a role in the mechanism of mutation fixation is indicated by a strikingly increased focusing of the mutations on intrinsic hot spots. We propose two phases to hypermutation targeting. The first is hot spot focused and MSH2 independent; the second, MSH2-dependent phase yields a more even spread of mutation fixation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • Binding Sites
  • Cell Line
  • DNA-Binding Proteins*
  • Female
  • Genes, Immunoglobulin*
  • Immunoglobulin Heavy Chains / genetics*
  • Male
  • Mice
  • Mice, Knockout
  • MutS Homolog 2 Protein
  • Mutation*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / physiology*


  • DNA-Binding Proteins
  • Immunoglobulin Heavy Chains
  • Proto-Oncogene Proteins
  • Msh2 protein, mouse
  • MutS Homolog 2 Protein