Effects of heat shock and hypoxia on protein synthesis in rainbow trout (Oncorhynchus mykiss) cells

J Exp Biol. 1998 Sep;201(Pt 17):2543-51.

Abstract

We examined the effects of heat stress (from 18 degreesC to 26 degreesC) and low oxygen tension (1% O2=1 kPa) on protein synthesis in primary cultures of hepatocytes, gill epithelial cells and fibroblast-like RTG-2 cells of rainbow trout Oncorhynchus mykiss. All these cell types displayed elevated levels of 67, 69 and 92 kDa proteins, whereas a 104 kDa protein was induced only in RTG-2 cells. Hypoxia induced a cell-type-specific response, increasing the synthesis of 36, 39 and 51 kDa proteins in the gill epithelial cells. The regulation of the heat-shock response in fish hepatocytes showed that an HSF1-like factor is involved in the transcriptional induction of the hsp70 gene. Consequently, there was a pronounced accumulation of hsp70 mRNA. Furthermore, the kinetics of activation of DNA binding and the increase in hsp70 gene expression showed a remarkable correlation, indicating that hsp70 expression is regulated at the transcriptional level in these trout cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Fibroblasts / metabolism
  • Gene Expression Regulation
  • Gills / cytology
  • Gills / metabolism
  • HSP70 Heat-Shock Proteins / biosynthesis*
  • Hot Temperature
  • Hypoxia / metabolism*
  • Liver / cytology
  • Liver / metabolism
  • Oncorhynchus mykiss / metabolism*
  • Protein Biosynthesis
  • Stress, Physiological / metabolism*

Substances

  • HSP70 Heat-Shock Proteins