An ethanolic-aqueous extract of Curcuma longa decreases the susceptibility of liver microsomes and mitochondria to lipid peroxidation in atherosclerotic rabbits

Biofactors. 1998;8(1-2):51-7. doi: 10.1002/biof.5520080110.


Atherosclerosis is characterized by oxidative damage which affects lipoproteins, the walls of blood vessels and subcellular membranes. This study evaluates the antioxidant capacity of a Curcuma longa extract on the lipid peroxidation of liver mitochondria and microsome membranes in atherosclerotic rabbits. Male rabbits fed a 3% (w/w) lard and 1.3% (w/w) cholesterol diet were randomly assigned to three groups. Two groups were treated with different dosages of a turmeric extract (A and B) and the third group (control) with a curcumin-free solution. Basal and in vitro 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hydroperoxide and TBARS productions in liver mitochondria and microsomes were analyzed. Group A had the lowest concentration of mitochondrial hydroperoxides. In microsomes, the basal hydroperoxide levels were similar in all groups but, after the induction of oxidation, group C registered the highest value; TBARS production followed the same trend in mitochondria. These findings suggest that active compounds in curcuma extract may be protective in preventing lipoperoxidation of subcellular membranes in a dosage-dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arteriosclerosis / metabolism*
  • Cholesterol, Dietary
  • Diet, Atherogenic
  • Dietary Fats
  • Ethanol
  • Lipid Peroxidation / drug effects
  • Lipid Peroxidation / physiology*
  • Male
  • Microsomes, Liver / drug effects*
  • Microsomes, Liver / metabolism
  • Mitochondria, Liver / drug effects*
  • Mitochondria, Liver / metabolism
  • Plant Extracts / pharmacology*
  • Rabbits
  • Thiobarbituric Acid Reactive Substances / analysis
  • Water


  • Cholesterol, Dietary
  • Dietary Fats
  • Plant Extracts
  • Thiobarbituric Acid Reactive Substances
  • Water
  • Ethanol
  • lard