Novel mechanism of action for Nur77 and antagonism by glucocorticoids: a convergent mechanism for CRH activation and glucocorticoid repression of POMC gene transcription

J Steroid Biochem Mol Biol. 1998 Apr;65(1-6):59-63. doi: 10.1016/s0960-0760(97)00180-5.

Abstract

Whereas orphan nuclear receptors of the Nur77 (NGFI-B) subfamily were previously known to act on transcription as monomers or as heterodimers with RXR, we have recently shown that Nur77 homodimers potently activate transcription upon interaction with a novel palindromic response element, the NurRE. In fact, reporter plasmids containing the NurRE respond to physiological stimuli in conditions where the NBRE, a binding site for Nur77 monomers, does not. Nur77 and its related receptors were shown to be important mediators for control of apoptosis induced by the T-cell receptor, and they also mediate the effect of the hypothalamic hormone CRH on transcription of the pituitary pro-opiomelanocotin (POMC) gene. In both systems, glucocorticoids antagonize the stimulatory effects of Nur77 on transcription by a mechanism that involves protein:protein interactions. Thus, the Nur77 signalling pathway appears to be a point of convergence for stimulatory signals and glucocorticoid repression in both endocrine and lymphoid systems.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Corticotropin-Releasing Hormone / pharmacology*
  • DNA-Binding Proteins / antagonists & inhibitors*
  • Dimerization
  • Endocrine System / metabolism
  • Gene Expression Regulation*
  • Glucocorticoids / pharmacology*
  • Lymphoid Tissue / metabolism
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Pro-Opiomelanocortin / biosynthesis*
  • Pro-Opiomelanocortin / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Steroid
  • Signal Transduction
  • Transcription Factors / antagonists & inhibitors*
  • Transcription, Genetic / drug effects

Substances

  • DNA-Binding Proteins
  • Glucocorticoids
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • Transcription Factors
  • Pro-Opiomelanocortin
  • Corticotropin-Releasing Hormone