SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors

Mol Cell. 1998 Jul;2(1):33-42. doi: 10.1016/s1097-2765(00)80111-2.


The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30, SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line, Transformed
  • DNA-Binding Proteins / physiology*
  • Histone Deacetylases / genetics
  • Histone Deacetylases / physiology*
  • Homeodomain Proteins / physiology*
  • Humans
  • Kidney
  • Macromolecular Substances
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Multiprotein Complexes
  • Nuclear Proteins / physiology*
  • Nuclear Receptor Co-Repressor 1
  • Receptors, Estrogen / antagonists & inhibitors
  • Receptors, Retinoic Acid / physiology
  • Receptors, Thyroid Hormone / physiology
  • Repressor Proteins / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factor Pit-1
  • Transcription Factors / physiology*
  • Transcription, Genetic*
  • Transfection


  • DNA-Binding Proteins
  • Homeodomain Proteins
  • Macromolecular Substances
  • Multiprotein Complexes
  • NCOR1 protein, human
  • Ncor1 protein, mouse
  • Nuclear Proteins
  • Nuclear Receptor Co-Repressor 1
  • POU1F1 protein, human
  • Pit1 protein, mouse
  • Receptors, Estrogen
  • Receptors, Retinoic Acid
  • Receptors, Thyroid Hormone
  • Repressor Proteins
  • SAP30 protein, human
  • SIN3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sap30 protein, mouse
  • Transcription Factor Pit-1
  • Transcription Factors
  • Histone Deacetylases

Associated data

  • GENBANK/AF075136