Involvement of CD4+ T cells in the development of dextran sulfate sodium-induced experimental colitis and suppressive effect of IgG on their action

Gen Pharmacol. 1998 Sep;31(3):477-81. doi: 10.1016/s0306-3623(98)00004-4.


1. To clarify the role of T cells in the development of dextran sulfate sodium (DSS)-induced colitis, T cells from colitis mice were primed with DSS-pulsed macrophages in vitro and then transferred into normal mice. In addition, to determine whether the target cell of immunoglobulin G (IgG) is the T cell, the extent of T cell proliferation induced by pulsed macrophages was examined after preincubation with IgG. 2. When mice receiving the primed T cells were treated with oral DSS, colitis symptoms were more severe than in animals treated with oral DSS only. This activity of primed T cells was reduced by depletion from the cells of CD4+ but not CD8+ cells. 3. The proliferation of T cells from colitis mice induced by pulsed macrophages was inhibited by T cell preincubation with homologous IgG. 4. The results suggest that CD4+ T cells play an important role in the development of DSS-induced experimental colitis and that IgG may modulate the development of colitis through interaction with pathogenic T cells.

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antilymphocyte Serum
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology
  • Cell Division / drug effects
  • Colitis / chemically induced
  • Colitis / immunology*
  • Dextran Sulfate / pharmacology*
  • Female
  • Immunoglobulin G / immunology*
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred CBA
  • T-Lymphocyte Subsets / immunology


  • Antilymphocyte Serum
  • Immunoglobulin G
  • Dextran Sulfate