Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts

Biochem Biophys Res Commun. 1998 Jul 30;248(3):450-3. doi: 10.1006/bbrc.1998.8988.


Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g., interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-2 by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-2 mRNA in human gingival fibroblasts.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins / biosynthesis*
  • Cells, Cultured
  • Culture Media, Conditioned
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Gene Expression Regulation / drug effects*
  • Gene Library
  • Gingiva / metabolism*
  • Humans
  • Interleukin-1 / pharmacology*
  • Osteoarthritis / metabolism
  • Protein Biosynthesis / drug effects
  • RNA, Messenger / biosynthesis
  • Synovial Membrane / metabolism*
  • Transcription, Genetic / drug effects
  • Transforming Growth Factor beta*


  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • Culture Media, Conditioned
  • Interleukin-1
  • RNA, Messenger
  • Transforming Growth Factor beta