Osteoprotegerin mRNA is increased by interleukin-1 alpha in the human osteosarcoma cell line MG-63 and in human osteoblast-like cells

Biochem Biophys Res Commun. 1998 Jul 30;248(3):696-700. doi: 10.1006/bbrc.1998.9035.


Osteoprotegerin (OPG) is a soluble receptor for the Osteoprotegerin-Ligand (OPGL) which is expressed on osteoblasts and mediates the signal for osteoclast differentiation. In the present study we demonstrate that OPG mRNA levels in MG-63 cells are increased in a dose-dependent manner after 8 h of treatment with IL-1 alpha (338 +/- 53% over control at 25 U/ml). Interleukin-6 (IL-6), under similar culture conditions, does not affect OPG mRNA levels. Time-course studies show that IL-1 alpha (25 U/ml) causes a transient increase of OPG mRNA levels in MG-63 cells, peaking after 4 h of treatment. An increase of the OPG transcript occurs in hOB cells at 0.5 h which is still present after 24 h of IL-1 alpha treatment. In MG-63 cells neither basal-nor IL-1 alpha-induced OPG mRNA levels are altered by the translational inhibitor cycloheximide. These results suggest that expression of OPG in osteoblasts may be regulated by IL-1 alpha.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Neoplasms
  • Cell Differentiation
  • Cycloheximide / pharmacology
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Glycoproteins / biosynthesis*
  • Humans
  • Interleukin-1 / pharmacology*
  • Kinetics
  • Osteoblasts / drug effects
  • Osteoblasts / metabolism*
  • Osteoclasts / cytology
  • Osteoprotegerin
  • Osteosarcoma
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • Receptors, Cytoplasmic and Nuclear*
  • Receptors, Tumor Necrosis Factor / biosynthesis
  • Signal Transduction
  • Transcription, Genetic / drug effects*
  • Tumor Cells, Cultured


  • Glycoproteins
  • Interleukin-1
  • Osteoprotegerin
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Tumor Necrosis Factor
  • TNFRSF11B protein, human
  • Cycloheximide