A missense mutation of the gene for Na+,K(+)-ATPase alpha-subunit causes abnormal feeding behavior in Caenorhabditis elegans

Biochem Biophys Res Commun. 1998 Jul 30;248(3):778-82. doi: 10.1006/bbrc.1998.8981.

Abstract

The Na+,K(+)-ATPase activity of membranes from a behavioral mutant of C. elegans was found to be about one-third that of the wild-type. The levels of mRNA and polypeptide of Na+,K(+)-ATPase alpha-subunit in the mutant were as high as those in the wild-type, but the level of the phosphorylated intermediate of the Na+,K(+)-ATPase in the mutant worm was 80% lower than that in the wild-type. A single predicted amino acid replacement (Leu to Phe) was found in a highly conserved region in the alpha-subunit that is involved in the formation of phosphorylated intermediate. The abnormal feeding behavior of the mutant worm may be attributed to this missense mutation.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / enzymology
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / physiology*
  • Cell Membrane / enzymology
  • Conserved Sequence
  • DNA, Helminth / genetics
  • Feeding Behavior*
  • Genes, Helminth*
  • Kinetics
  • Macromolecular Substances
  • Molecular Sequence Data
  • Ouabain / pharmacology
  • Phosphorylation
  • Point Mutation*
  • Protein Biosynthesis
  • RNA, Messenger / biosynthesis
  • Sodium-Potassium-Exchanging ATPase / chemistry
  • Sodium-Potassium-Exchanging ATPase / genetics*
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Transcription, Genetic

Substances

  • DNA, Helminth
  • Macromolecular Substances
  • RNA, Messenger
  • Ouabain
  • Sodium-Potassium-Exchanging ATPase

Associated data

  • GENBANK/AB002154