Cloning and expression of human UDP-glucuronosyltransferase (UGT) 1A8

Arch Biochem Biophys. 1998 Aug 15;356(2):301-5. doi: 10.1006/abbi.1998.0781.


The mRNA expression of human UDP-glucuronosyltransferase 1A8 (UGT1A8) has been found in jejunum, ileum, and colon but not in liver. A cDNA with a complete UGT1A8 coding region was amplified from total human ileal RNA by reverse transcriptase-polymerase chain reaction and inserted into the mammalian expression vector, pcDNA3. Lysates of HK293 cells expressing UGT1A8 revealed the expression of a protein with a molecular mass of 56 kDa by Western blot analysis. Transiently expressed human UGT1A8 shows glucuronidation activities with coumarins, anthraquinones, flavonoids, phenolic compounds, catechol estrogens, 17-hydroxyandrogens, primary amines such as the carcinogen 4-aminobiphenyl, and certain opioids. This UGT may play an important role in the detoxification of xenobiotics in human intestine.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Catalysis
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • Enzyme Activation
  • Gene Expression Regulation, Enzymologic*
  • Glucuronosyltransferase / biosynthesis*
  • Glucuronosyltransferase / genetics*
  • Glucuronosyltransferase / metabolism
  • Humans
  • Kidney / cytology
  • Organ Specificity / genetics
  • RNA, Messenger / metabolism
  • Transfection


  • DNA, Complementary
  • RNA, Messenger
  • Glucuronosyltransferase