Conserved evolution of the Rh50 gene compared to its homologous Rh blood group gene

Biochem Biophys Res Commun. 1998 Aug 10;249(1):78-85. doi: 10.1006/bbrc.1998.9074.


We have sequenced the complete coding region of the Rh blood group gene for mouse and rat and that of Rh-related 50 kD glycoprotein (Rh50) for mouse, rat, and crab-eating macaque. Phylogenetic analyses of Rh and Rh50 amino acid sequences indicate that the Rh50 gene has been evolving about two times more slowly than the Rh blood group gene in both primates and rodents. This conservative nature of the Rh50 gene suggests its relative importance to the Rh blood group gene. The time of gene duplication that produced the Rh and Rh50 genes was estimated to be about 240-310 million years ago. We also conducted window analyses of synonymous and nonsynonymous nucleotide substitutions for those two genes. Some peaks where nonsynonymous substitutions are higher than synonymous ones were located on outer membrane regions. This suggests the existence of positive Darwinian selection on Rh and Rh50 genes through host-parasite interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blood Proteins / genetics*
  • Conserved Sequence*
  • Evolution, Molecular*
  • Glycoproteins / genetics*
  • Humans
  • Macaca
  • Membrane Glycoproteins*
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • Rats
  • Rh-Hr Blood-Group System / genetics*
  • Sequence Analysis, DNA


  • Blood Proteins
  • Glycoproteins
  • Membrane Glycoproteins
  • RHAG protein, human
  • Rh-Hr Blood-Group System
  • Rhag protein, mouse
  • Rhag protein, rat

Associated data

  • GENBANK/AB015189
  • GENBANK/AB015190
  • GENBANK/AB015191
  • GENBANK/AB015192
  • GENBANK/AB015193
  • GENBANK/AB015194
  • GENBANK/AB015467