Immune response of sows vaccinated with attenuated transmissible gastroenteritis virus (TGEV) and recombinant TGEV spike protein vaccines and protection of their suckling pigs against virulent TGEV challenge exposure

Am J Vet Res. 1998 Aug;59(8):1002-8.


Objective: To compare recombinant transmissible gastroenteritis virus (TGEV) spike protein, (SP) R2-2, with attenuated live virus (ALV) vaccine in sows during late pregnancy.

Animals: 13 TGEV-seronegative sows and their pigs.

Procedure: At prepartum weeks (PPW) 6 and 4, sows of groups 1 and 2 received ALV via the oral/intranasal (O/IN) route. At PPW 2, group-1 sows received ALV IM and group-2 sows received SPR2-2 IM. Group-3 sows received SPR2-2 IM at PPW 4 and ALV O/IN at PPW 2. Sows of group 4 (negative controls) were inoculated O/IN with mock-infected ST cell fluids at PPW 6 and 4 and IM with Sf9 cell lysates at PPW2 (n = 2), or IM with Sf9 cell lysates at PPW4 and O/IN with mock-infected ST cell fluids at PPW2 (2). Serum, colostrum, and milk samples were tested for antibody to TGEV, and a lymphoproliferative (LP) assay was done on blood mononuclear cells. Suckling pigs were challenge exposed with virulent TGEV.

Results: Sows of groups 1 and 2 had higher IgG and significantly higher antibody titers in colostrum; their pigs had significantly higher serum antibody titer. At challenge exposure of their pigs, LP responses of group-2 sows were significantly higher than those of sows in the other 3 groups. Mean pig mortality ranged from 43 (group 2) to 92% (group 4). Significant negative correlations were observed among litter mortality and sow LP response, colostral titer, and pig serum titer at time of challenge exposure.

Conclusions: In sows vaccinated twice with attenuated live TGEV, the recombinant SPR2-2 administered IM may be comparable to ALV administered IM as a booster. Vaccination failed to provide complete protection to suckling pigs after challenge exposure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Antibody Formation
  • Antigens, Viral / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Feces / virology
  • Female
  • Gastroenteritis, Transmissible, of Swine / immunology*
  • Gastroenteritis, Transmissible, of Swine / prevention & control
  • Immunoglobulin A / blood
  • Immunoglobulin G / blood
  • Pregnancy
  • Pregnancy Complications, Infectious / immunology
  • Pregnancy Complications, Infectious / prevention & control
  • Pregnancy Complications, Infectious / veterinary*
  • Pregnancy, Animal / immunology*
  • Swine
  • Transmissible gastroenteritis virus / immunology*
  • Vaccines, Attenuated*
  • Vaccines, Synthetic*
  • Viral Proteins / immunology*
  • Viral Vaccines*


  • Antibodies, Viral
  • Antigens, Viral
  • Immunoglobulin A
  • Immunoglobulin G
  • Vaccines, Attenuated
  • Vaccines, Synthetic
  • Viral Proteins
  • Viral Vaccines
  • spike protein S, Transmissible gastroenteritis virus