p53 Alterations in Betel Quid- And Tobacco-Associated Oral Squamous Cell Carcinomas From Taiwan

J Oral Pathol Med. 1998 Jul;27(6):243-8. doi: 10.1111/j.1600-0714.1998.tb01950.x.

Abstract

Alterations of p53 have been explored in Taiwanese oral squamous cell carcinomas (OSCCs) consisting of a betel quid (BQ)/tobacco-related subgroup of 36 subjects and a tobacco-related subgroup of 13 subjects. Mutations in conserved exons were found in 12 tumors. Seven mutations were clustered in a hot-spot region mapped to a region between codons 273-282 in exon 8. The incidence of p53 mutation in BQ/tobacco tumors was 22% (8/36). The frequency of p53 allelic loss (21%, 3/14) in BQ/tobacco tumors approximates to the incidence of mutation. This is the first study demonstrating allelic deletion of p53 in such malignancies. Twenty-four of 43 samples showed positive p53 immunostaining. All tumors harboring mis-sense mutations of p53 in conserved exons exhibited nuclear protein accumulation. The incidence of mutation in conserved exons in BQ/tobacco-associated Asian OSCCs (15%) is significantly different from worldwide OSCCs (46%) related primarily to tobacco consumption (P=0.00001).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alleles
  • Areca*
  • Carcinoma, Squamous Cell / genetics*
  • Codon / genetics
  • Coloring Agents
  • Exons / genetics
  • Female
  • Gene Deletion
  • Gene Frequency
  • Genes, p53 / genetics*
  • Humans
  • Immunohistochemistry
  • Incidence
  • Loss of Heterozygosity / genetics
  • Male
  • Middle Aged
  • Mouth Neoplasms / genetics*
  • Mutation / genetics
  • Nuclear Proteins / genetics
  • Plants, Medicinal*
  • Smoking / adverse effects*
  • Taiwan

Substances

  • Codon
  • Coloring Agents
  • Nuclear Proteins