lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal

Cell. 1998 Aug 7;94(3):287-97. doi: 10.1016/s0092-8674(00)81472-5.

Abstract

lefty-1, lefty-2, and nodal are expressed on the left side of developing mouse embryos and are implicated in left-right (L-R) determination. The role of lefty-1 was examined by analyzing mutant mice lacking this gene. The lefty-1-deficient mice showed a variety of L-R positional defects in visceral organs. Unexpectedly, however, the most common feature of lefty-1-/- mice was thoracic left isomerism (rather than right isomerism). The lack of lefty-1 resulted in bilateral expression of nodal, lefty-2, and Pitx2 (a homeobox gene normally expressed on the left side). These observations suggest that the role of lefty-1 is to restrict the expression of lefty-2 and nodal to the left side, and that lefty-2 or nodal encodes a signal for "leftness."

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Body Patterning / genetics*
  • Gene Expression Regulation, Developmental*
  • Homeodomain Proteins / biosynthesis
  • Homeodomain Proteins / genetics
  • Left-Right Determination Factors
  • Lung / abnormalities
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Mutation
  • Nodal Protein
  • Nuclear Proteins*
  • Paired Box Transcription Factors
  • Proteins / genetics*
  • Proteins / physiology*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Transforming Growth Factor beta / deficiency
  • Transforming Growth Factor beta / genetics*
  • Transforming Growth Factor beta / physiology*

Substances

  • Homeodomain Proteins
  • Left-Right Determination Factors
  • Nodal Protein
  • Nodal protein, mouse
  • Nuclear Proteins
  • Paired Box Transcription Factors
  • Proteins
  • Transcription Factors
  • Transforming Growth Factor beta
  • homeobox protein PITX1
  • homeobox protein PITX3
  • homeobox protein PITX2