The Meiosis-Specific Hop2 Protein of S. Cerevisiae Ensures Synapsis Between Homologous Chromosomes

Cell. 1998 Aug 7;94(3):375-86. doi: 10.1016/s0092-8674(00)81480-4.

Abstract

The hop2 mutant of S. cerevisiae displays a novel phenotype: meiotic chromosomes form nearly wild-type amounts of synaptonemal complex, but most chromosomes are engaged in synapsis with nonhomologous partners. The meiosis-specific Hop2 protein localizes to chromosomes prior to and during synapsis and in the absence of the double-strand breaks that initiate recombination. hop2 strains sustain a wild-type level of meiotic double-strand breaks, but these breaks remain unrepaired. The hop2 mutant arrests at the pachytene stage of meiotic prophase with the RecA-like protein Dmc1 located at numerous sites along synapsed chromosomes. We propose that the Hop2 protein functions to prevent synapsis between nonhomologous chromosomes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle Proteins*
  • Chromosomes / genetics*
  • Cloning, Molecular
  • DNA Damage
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / chemistry*
  • Fungal Proteins / genetics*
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / physiology*
  • Gene Conversion
  • Gene Expression Regulation, Fungal
  • Meiosis / genetics*
  • Molecular Sequence Data
  • Mutation
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Synaptonemal Complex / genetics

Substances

  • Cell Cycle Proteins
  • DMC1 protein, S cerevisiae
  • DNA-Binding Proteins
  • Fungal Proteins
  • HOP2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins

Associated data

  • GENBANK/AF078740