Functional expression of human P-glycoprotein from plasmids using vaccinia virus-bacteriophage T7 RNA polymerase system

Methods Enzymol. 1998;292:456-73. doi: 10.1016/s0076-6879(98)92035-3.
No abstract available

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / isolation & purification
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism
  • Adenosine Triphosphatases / metabolism
  • Affinity Labels
  • Azides / pharmacokinetics
  • Cell Membrane / metabolism
  • Cloning, Molecular / methods
  • Colchicine / pharmacokinetics
  • DNA-Directed RNA Polymerases*
  • Daunorubicin / pharmacokinetics
  • Dihydropyridines / pharmacokinetics
  • Drug Resistance, Multiple
  • Electrophoresis, Polyacrylamide Gel / methods
  • Escherichia coli
  • HeLa Cells
  • Humans
  • Indicators and Reagents
  • Iodine Radioisotopes
  • Osteosarcoma
  • Plasmids
  • Polymerase Chain Reaction / methods
  • Prazosin / analogs & derivatives
  • Prazosin / pharmacokinetics
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Transfection / methods
  • Tritium
  • Tumor Cells, Cultured
  • Vaccinia virus*
  • Vinblastine / pharmacokinetics
  • Viral Proteins

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Affinity Labels
  • Azides
  • Dihydropyridines
  • Indicators and Reagents
  • Iodine Radioisotopes
  • Recombinant Proteins
  • Viral Proteins
  • Tritium
  • Vinblastine
  • azidopine
  • azidoprazosin
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases
  • Adenosine Triphosphatases
  • Colchicine
  • Prazosin
  • Daunorubicin