Overexpression, purification, and function of first nucleotide-binding fold of cystic fibrosis transmembrane conductance regulator

Methods Enzymol. 1998;292:675-86. doi: 10.1016/s0076-6879(98)92052-3.
No abstract available

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Adenosine Triphosphate / metabolism*
  • Binding Sites
  • Carrier Proteins / biosynthesis
  • Circular Dichroism
  • Cloning, Molecular / methods
  • Cystic Fibrosis Transmembrane Conductance Regulator / biosynthesis
  • Cystic Fibrosis Transmembrane Conductance Regulator / chemistry*
  • Cystic Fibrosis Transmembrane Conductance Regulator / physiology*
  • DNA Primers
  • Escherichia coli
  • Escherichia coli Proteins*
  • Genetic Vectors
  • Humans
  • Maltose-Binding Proteins
  • Monosaccharide Transport Proteins*
  • Mutagenesis
  • Polymerase Chain Reaction / methods
  • Protein Folding*
  • Protein Structure, Secondary*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Sequence Deletion

Substances

  • ATP-Binding Cassette Transporters
  • CFTR protein, human
  • Carrier Proteins
  • DNA Primers
  • Escherichia coli Proteins
  • Maltose-Binding Proteins
  • Monosaccharide Transport Proteins
  • Recombinant Fusion Proteins
  • maltose transport system, E coli
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Adenosine Triphosphate