In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system

Methods Enzymol. 1998;293:504-29. doi: 10.1016/s0076-6879(98)93031-2.

Abstract

A general method for the incorporation of unnatural amino acids into ion channels and membrane receptors using a Xenopus oocyte expression system has been described. A large number of unnatural amino acids have been incorporated into the nAChR, GIRK, and Shaker K+ channels. Continuing efforts focus on incorporating unnatural amino acids that differ substantially from the natural amino acids, for example, residues that include fluorophores. In addition, we are addressing the feasibility of incorporating unnatural amino acids into ion channels and membrane receptors in mammalian cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / metabolism*
  • Animals
  • Base Sequence
  • Codon
  • Female
  • Gene Expression
  • In Vitro Techniques
  • Ion Channels / biosynthesis*
  • Ion Channels / chemistry
  • Molecular Sequence Data
  • Molecular Structure
  • Nucleic Acid Conformation
  • Oligodeoxyribonucleotides
  • Oocytes / physiology*
  • Point Mutation
  • Potassium Channels / biosynthesis*
  • Potassium Channels / chemistry
  • Promoter Regions, Genetic
  • RNA, Transfer, Gln / chemistry
  • RNA, Transfer, Gln / genetics*
  • RNA, Transfer, Gln / metabolism
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / chemistry
  • Receptors, Nicotinic / biosynthesis
  • Receptors, Nicotinic / chemistry
  • Shaker Superfamily of Potassium Channels
  • Suppression, Genetic
  • Thermus thermophilus / genetics
  • Transcription, Genetic
  • Xenopus laevis

Substances

  • Amino Acids
  • Codon
  • Ion Channels
  • Oligodeoxyribonucleotides
  • Potassium Channels
  • RNA, Transfer, Gln
  • Receptors, Cell Surface
  • Receptors, Nicotinic
  • Shaker Superfamily of Potassium Channels