Characterization of lymphocytic subsets and cytokine production in gastric biopsy samples from Helicobacter pylori patients

Scand J Gastroenterol. 1998 Jul;33(7):704-9. doi: 10.1080/00365529850171639.


Background: This study characterized the phenotypic subsets of isolated gastric lymphocytes and the cellular immune response in cultured gastric biopsy specimens.

Methods: Endoscopy specimens from 40 Helicobacter pylori-positive and 40 H. pylori-negative patients were studied. a) Isolated gastric lymphocytes were analysed for CD4+, CD8+ T-lymphocyte subsets, activated T cells, and natural killer cells on a fluorescence-activated cell sorter, using monoclonal antibodies. b) The supernatant of cultured gastric biopsy specimens were assayed for interleukin (IL)-2, IL-4, and IL-6 levels.

Results: In H. pylori-positive patients there was (a) a decrease in CD4+/CD8+ T cells, no change in activated T cells, and an increase in natural killer cells, and (b) no change in IL-2 levels and a significant increase in IL-4 and IL-6 levels.

Conclusions: There is an increase in CD8+ lymphocytes and natural killer cells, and the observed increase in IL-4 and IL-6 might be important in H. pylori-associated gastritis.

Publication types

  • Clinical Trial
  • Controlled Clinical Trial

MeSH terms

  • Adult
  • Aged
  • Biopsy, Needle
  • CD4-CD8 Ratio
  • Cells, Cultured
  • Chronic Disease
  • Cytokines / biosynthesis*
  • Dyspepsia / etiology
  • Dyspepsia / physiopathology
  • Female
  • Gastric Mucosa / metabolism*
  • Gastric Mucosa / microbiology
  • Gastric Mucosa / pathology
  • Gastritis / metabolism*
  • Gastritis / microbiology
  • Gastritis / pathology
  • Gastroscopy
  • Helicobacter Infections / physiopathology*
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Immunity, Cellular / immunology
  • Male
  • Middle Aged
  • Sensitivity and Specificity
  • Statistics, Nonparametric
  • T-Lymphocyte Subsets / metabolism*


  • Cytokines