Hepatoprotective effect of C-phycocyanin: protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicty in rats

Biochem Biophys Res Commun. 1998 Aug 19;249(2):428-31. doi: 10.1006/bbrc.1998.9149.

Abstract

Effect of C-phycocyanin (from Spirulina platensis) pretreatment on carbontetrachloride and R-(+)-pulegone-induced hepatotoxicity in rats was studied. Intraperitoneal (i.p.) administration (200 mg/kg) of a single dose of phycocyanin to rats, one or three hours prior to R-(+)-pulegone (250 mg/kg) or carbontetrachloride (0.6 ml/kg) challenge, significantly reduced the hepatotoxicity caused by these chemicals. For instance, serum glutamate pyruvate transaminase (SGPT) activity was almost equal to control values. The losses of microsomal cytochrome P450, glucose-6-phosphatase and aminopyrine-N-demethylase were significantly reduced, suggesting that phycocyanin provides protection to liver enzymes. It was noticed that the level of menthofuran, the proximate toxin of R-(+)-pulegone was nearly 70% more in the urine samples collected from rats treated with R-(+)-pulegone alone than rats treated with the combination of phycocyanin and R-(+)-pulegone. The possible mechanism involved in the hepatoprotection is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine Transaminase / blood
  • Aminopyrine N-Demethylase / metabolism
  • Animals
  • Carbon Tetrachloride / toxicity*
  • Chemical and Drug Induced Liver Injury*
  • Cyclohexane Monoterpenes
  • Cytochrome P-450 Enzyme System / metabolism
  • Glucose-6-Phosphatase / metabolism
  • Liver / enzymology
  • Liver Diseases / enzymology
  • Liver Diseases / prevention & control*
  • Male
  • Menthol / analogs & derivatives
  • Menthol / toxicity
  • Menthol / urine
  • Microsomes, Liver / enzymology
  • Monoterpenes*
  • Phycocyanin / therapeutic use*
  • Rats
  • Terpenes / urine

Substances

  • Cyclohexane Monoterpenes
  • Monoterpenes
  • Terpenes
  • Phycocyanin
  • Menthol
  • menthofuran
  • pulegone
  • Cytochrome P-450 Enzyme System
  • Carbon Tetrachloride
  • Aminopyrine N-Demethylase
  • Alanine Transaminase
  • Glucose-6-Phosphatase