Expression of metastasis-related genes in surgical specimens of human gastric cancer can predict disease recurrence

Eur J Cancer. 1998 Mar;34(4):558-65. doi: 10.1016/s0959-8049(97)10075-2.


It was determined whether the expression level of several genes that regulate different steps of metastasis in formalin-fixed paraffin-embedded archival specimens of human gastric cancers correlated with disease recurrence and metastasis. The steady-state mRNA expression level for epidermal growth factor receptor (EGF-R), basic fibroblast growth factor (bFGF), E-cadherin, type IV collagenase and multidrug resistance (MDR-1) were examined by a colorimetric in situ hybridisation (ISH) technique, concentrating on reactivity at the periphery of the lesions. All patients were operated on for cure. 15 cases were disease-free and 10 had disease recurrence by 4.5 years after resection of the primary tumours. The expression of EGF-R and bFGF type IV collagenase was higher and expression of E-cadherin was lower in the disease-recurrence cases than in the disease-free cases. The ratio between the expression level of collagenase type IV and E-cadherin at the periphery of the surgical specimens differed significantly between the disease-free cases and the recurrent-metastatic cases. These data show that multiparametric ISH analysis for several metastasis-related genes may allow prediction of disease recurrence of gastric cancer.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cadherins / genetics
  • Cadherins / metabolism
  • Collagenases / genetics
  • Collagenases / metabolism
  • ErbB Receptors / genetics
  • ErbB Receptors / metabolism
  • Fibroblast Growth Factor 2 / genetics
  • Fibroblast Growth Factor 2 / metabolism
  • Gene Expression
  • Genes, MDR
  • Humans
  • In Situ Hybridization
  • Matrix Metalloproteinase 9
  • Neoplasm Recurrence, Local / diagnosis*
  • Neoplasm Recurrence, Local / metabolism
  • RNA, Messenger / metabolism*
  • RNA, Neoplasm / metabolism*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism


  • Cadherins
  • RNA, Messenger
  • RNA, Neoplasm
  • Fibroblast Growth Factor 2
  • ErbB Receptors
  • Collagenases
  • Matrix Metalloproteinase 9