Effects of D-glucose and starvation upon the cyclic ADP-ribose content of rat pancreatic islets

Biochem Mol Biol Int. 1998 Jul;45(4):783-90. doi: 10.1080/15216549800203202.


Rat pancreatic islets were found to display a much lower content of immunoreactive CD38 and a much lower ADP-ribosyl cyclase activity than rat spleen or brain. Cyclic ADP-ribose was also measured by a radioimmunological procedure in rat pancreatic islets. In fed rats, the cyclic ADP-ribose content appeared higher after isolation of the islets in the presence of 2.8 mM D-glucose rather than in the absence of the hexose, progressively increased during incubation of the islets for 5-60 min at 37 degrees C, but failed to be affected by the concentration of D-glucose (zero to 20.0 mM) in the incubation medium. In rats fasted for 24 hours, the cyclic ADP-ribose islet content also increased during incubation, but again failed to be affected by the concentration of D-glucose in the incubation medium. Although these findings indicate that the islet cyclic ADP-ribose content is influenced by nutritional and environmental factors, they do not support the view that the insulinotropic action of D-glucose involves major change in the islet cell content of the cyclic nucleotide.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase
  • ADP-ribosyl Cyclase 1
  • Adenosine Diphosphate Ribose / analogs & derivatives*
  • Adenosine Diphosphate Ribose / metabolism
  • Animals
  • Antigens, CD*
  • Antigens, Differentiation / metabolism
  • Brain / metabolism
  • Cell Separation
  • Cyclic ADP-Ribose
  • Fasting
  • Glucose / pharmacology*
  • Islets of Langerhans / metabolism*
  • Membrane Glycoproteins
  • NAD+ Nucleosidase / metabolism
  • Rats
  • Spleen / metabolism
  • Starvation*


  • Antigens, CD
  • Antigens, Differentiation
  • Membrane Glycoproteins
  • Cyclic ADP-Ribose
  • Adenosine Diphosphate Ribose
  • ADP-ribosyl Cyclase
  • Cd38 protein, rat
  • NAD+ Nucleosidase
  • ADP-ribosyl Cyclase 1
  • Glucose