Proliferative T-cell responses to human papillomavirus type 16 E5 are decreased amongst women with high-grade neoplasia

J Gen Virol. 1998 Aug;79 ( Pt 8):1971-6. doi: 10.1099/0022-1317-79-8-1971.

Abstract

Proliferative responses to human papillomavirus type 16 (HPV-16) E5 peptides were determined for short-term cell lines derived from peripheral blood mononuclear cells of 75 women. Cell lines from 16 of the 75 women proliferated in response to stimulation with pooled E5 peptides; this was most common for patients with low-grade squamous cervical intraepithelial lesions (LSIL; 6 of 15 patients, 40%) and less frequent for asymptomatic women with no cervical lesions (4 of 20, 20%), those with high-grade squamous intraepithelial lesions(HSIL; 5 of 33, 15%) and others with cervical cancer (1 of 7, 14%, P = 0.027). Amongst these patients, proliferative responses were exclusive to those that were positive for HPV-16 DNA (12 of 41, 29%; c.f. none of 13 HPV-16 DNA-negative subjects exhibited a proliferative response; P= 0023) and were again most prevalent amongst HPV-16 DNA-positive LSIL (6 of 14, 43%), as compared with HPV-16 DNA-positive HSIL (5 of 23, 22%) or HPV-16 DNA-positive cervical cancer patients (1 of 4, 25%, P > 0.05). In contrast, for asymptomatic women, responsiveness was statistically independent of HPV-16 DNA status, i.e. responsiveness in HPV-16 DNA-positive and DNA-negative subjects was observed in 3 of 15 (20%) and 1 of 5 (20%) cases, respectively (P > 0.05). There were no associations between detection of HPV-16 mRNA and proliferative responses (P> 0.05). These data suggest that HPV-16 E5-specific T-helper activity is depressed amongst women with HSIL lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Division
  • Cells, Cultured
  • Cervical Intraepithelial Neoplasia / blood
  • Cervical Intraepithelial Neoplasia / immunology*
  • Cervical Intraepithelial Neoplasia / virology
  • Female
  • Humans
  • Middle Aged
  • Neoplasms, Squamous Cell / blood
  • Neoplasms, Squamous Cell / immunology*
  • Neoplasms, Squamous Cell / virology
  • Oncogene Proteins, Viral / immunology*
  • Papillomaviridae / genetics
  • Papillomaviridae / immunology*
  • Papillomavirus Infections / blood
  • Papillomavirus Infections / immunology*
  • Papillomavirus Infections / virology
  • Peptides / chemical synthesis
  • Peptides / immunology
  • T-Lymphocytes / immunology*
  • Tumor Virus Infections / blood
  • Tumor Virus Infections / immunology*
  • Tumor Virus Infections / virology
  • Uterine Cervical Neoplasms / blood
  • Uterine Cervical Neoplasms / immunology*
  • Uterine Cervical Neoplasms / virology

Substances

  • Oncogene Proteins, Viral
  • Peptides
  • oncogene protein E5, Human papillomavirus type 16