Abstract
We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-terminus. Pex11p comigrated in SDS-PAGE with a 28-kDa peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N- and C-terminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies / immunology
-
Base Sequence
-
CHO Cells
-
Clofibrate / pharmacology*
-
Cloning, Molecular
-
Cricetinae
-
DNA Primers
-
DNA, Complementary
-
Fungal Proteins / genetics*
-
Fungal Proteins / immunology
-
Gene Expression Regulation / drug effects*
-
Humans
-
Male
-
Membrane Proteins / genetics*
-
Membrane Proteins / immunology
-
Microbodies / metabolism*
-
Molecular Sequence Data
-
Peroxins
-
Rats
-
Rats, Inbred F344
-
Saccharomyces cerevisiae Proteins*
-
Sequence Homology, Amino Acid
Substances
-
Antibodies
-
DNA Primers
-
DNA, Complementary
-
Fungal Proteins
-
Membrane Proteins
-
PEX11 protein, S cerevisiae
-
PEX11A protein, human
-
PEX11B protein, human
-
Peroxins
-
Pex11a protein, rat
-
Saccharomyces cerevisiae Proteins
-
Clofibrate