Clofibrate-inducible, 28-kDa peroxisomal integral membrane protein is encoded by PEX11

FEBS Lett. 1998 Jul 24;431(3):468-72. doi: 10.1016/s0014-5793(98)00815-1.


We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-terminus. Pex11p comigrated in SDS-PAGE with a 28-kDa peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N- and C-terminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Base Sequence
  • CHO Cells
  • Clofibrate / pharmacology*
  • Cloning, Molecular
  • Cricetinae
  • DNA Primers
  • DNA, Complementary
  • Fungal Proteins / genetics*
  • Fungal Proteins / immunology
  • Gene Expression Regulation / drug effects*
  • Humans
  • Male
  • Membrane Proteins / genetics*
  • Membrane Proteins / immunology
  • Microbodies / metabolism*
  • Molecular Sequence Data
  • Peroxins
  • Rats
  • Rats, Inbred F344
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid


  • Antibodies
  • DNA Primers
  • DNA, Complementary
  • Fungal Proteins
  • Membrane Proteins
  • PEX11 protein, S cerevisiae
  • PEX11A protein, human
  • PEX11B protein, human
  • Peroxins
  • Pex11a protein, rat
  • Saccharomyces cerevisiae Proteins
  • Clofibrate

Associated data

  • GENBANK/AB015594