Digital imaging microscopy of living cells

Trends Cell Biol. 1998 Jul;8(7):288-92. doi: 10.1016/s0962-8924(98)01301-4.


Fluorescence microscopy has undergone a resurgence in interest following the discovery of green-fluorescent protein (GFP) and its increasing use in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be used to produce high-resolution three-dimensional images of samples labelled with GFP, or other fluorochromes, using simple instrumentation and image-restoration software.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Cell Physiological Phenomena*
  • Image Processing, Computer-Assisted / methods*
  • Microscopy, Confocal / instrumentation
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*