Intranasal administration of HIV-DNA vaccine formulated with a polymer, carboxymethylcellulose, augments mucosal antibody production and cell-mediated immune response

Clin Immunol Immunopathol. 1998 Aug;88(2):205-10. doi: 10.1006/clin.1998.4566.


We previously reported that intramuscular (i.m.) immunization of DNA vaccine encoding human immunodeficiency virus type 1 (HIV-1)IIIB env and rev genes alone or in combination with appropriate adjuvant induces substantial and enhanced immune response against HIV-1. In the present study, we examined whether a polymer, low-viscosity carboxymethylcellulose sodium salt (CMCS-L), has an adjuvant effect on immune response induced by DNA vaccination. BALB/c mice were immunized with HIV-DNA vaccine formulated with CMCS-L via the intranasal (i.n.) and i.m. routes. The combination with the polymer elicited higher levels of antigen-specific serum IgG and fecal IgA antibodies than DNA vaccine alone. For cell-mediated immunity, HIV-specific delayed-type hypersensitivity response and cytotoxic T lymphocyte activity were measured by the footpad-swelling test and the 51Cr-release assay, respectively. Both were enhanced by the combination with CMCS-L via i.n. and i.m. inoculation. Cytokine analysis in culture media of bulk splenocytes harvested from immunized animals showed higher levels of IL-4 production in i.n. -immunized mice compared with i.m.-immunized mice. Nevertheless, the increased IFN-gamma production resulting from the combination with CMCS-L was observed only in i.n.-immunized mice. These data indicate that i.n. immunization of HIV-DNA vaccine formulated with CMCS-L enhances HIV-specific mucosal antibody (Ab) and systemic Ab and cell-mediated immune response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / immunology*
  • AIDS Vaccines / pharmacology*
  • Adjuvants, Immunologic / pharmacology*
  • Administration, Intranasal
  • Animals
  • Antibodies / blood
  • Carboxymethylcellulose Sodium / pharmacology*
  • Cells, Cultured
  • Female
  • HIV / genetics*
  • HIV / immunology*
  • Hypersensitivity, Delayed
  • Immunity, Cellular / drug effects*
  • Immunity, Mucosal / drug effects*
  • Immunoglobulin G / classification
  • Injections, Intramuscular
  • Interferon-gamma / biosynthesis
  • Interleukin-4 / biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Polymers / pharmacology*
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / metabolism
  • Titrimetry
  • Vaccines, DNA / immunology*
  • Vaccines, DNA / pharmacology*


  • AIDS Vaccines
  • Adjuvants, Immunologic
  • Antibodies
  • Immunoglobulin G
  • Polymers
  • Vaccines, DNA
  • Interleukin-4
  • Interferon-gamma
  • Carboxymethylcellulose Sodium