Cloning of a Novel Member of the Low-Density Lipoprotein Receptor Family

Gene. 1998 Aug 17;216(1):103-11. doi: 10.1016/s0378-1119(98)00311-4.

Abstract

A gene encoding a novel transmembrane protein was identified by DNA sequence analysis within the insulin-dependent diabetes mellitus (IDDM) locus IDDM4 on chromosome 11q13. Based on its chromosomal position, this gene is a candidate for conferring susceptibility to diabetes. The gene, termed low-density lipoprotein receptor related protein 5 (LRP5), encodes a protein of 1615 amino acids that contains conserved modules which are characteristic of the low-density lipoprotein (LDL) receptor family. These modules include a putative signal peptide for protein export, four epidermal growth factor (EGF) repeats with associated spacer domains, three LDL-receptor (LDLR) repeats, a single transmembrane spanning domain, and a cytoplasmic domain. The encoded protein has a unique organization of EGF and LDLR repeats; therefore, LRP5 likely represents a new category of the LDLR family. Both human and mouse LRP5 cDNAs have been isolated and the encoded mature proteins are 95% identical, indicating a high degree of evolutionary conservation.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosomes, Human, Pair 11 / genetics
  • Cloning, Molecular
  • Cosmids / genetics
  • DNA / chemistry
  • DNA / genetics
  • DNA, Bacterial / genetics
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Diabetes Mellitus, Type 1 / genetics
  • Gene Expression / genetics
  • Genetic Predisposition to Disease
  • Genomic Library
  • Humans
  • LDL-Receptor Related Proteins
  • Low Density Lipoprotein Receptor-Related Protein-5
  • Membrane Proteins / genetics
  • Mice
  • Microsatellite Repeats / genetics
  • Molecular Sequence Data
  • Receptors, LDL / genetics*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • DNA, Bacterial
  • DNA, Complementary
  • LDL-Receptor Related Proteins
  • LRP5 protein, human
  • Low Density Lipoprotein Receptor-Related Protein-5
  • Lrp5 protein, mouse
  • Membrane Proteins
  • Receptors, LDL
  • DNA

Associated data

  • GENBANK/AF064548
  • GENBANK/AF064984