Cloning and characterization of an actin-resistant DNase I-like endonuclease secreted by macrophages

Gene. 1998 Jul 30;215(2):291-301. doi: 10.1016/s0378-1119(98)00281-9.

Abstract

We have cloned human and murine DNase I-like cDNAs, termed LS-DNase, which are expressed at high levels in liver and spleen tissues. LS-DNase expression is highly specific to macrophage populations within these and other tissues. Mature LS-DNase from both species is a secreted, non-glycosylated protein containing 285 residues, with a calculated molecular mass of 33 kDa and a basic isoelectric point. Human and murine LS-DNase are highly conserved and share 83% identity. Sequence analysis reveals that LS-DNase shares 46% amino acid sequence identity with DNase I. However, several residues identified as important for interaction of human DNase I with actin are not conserved in both human and murine LS-DNase. Consistent with this observation, recombinant human LS-DNase possesses a DNA hydrolytic activity which, unlike DNase I, is not inhibited by G-actin. The existence of a family of DNase I-like molecules that have tissue-specific expression patterns and the possible role of a macrophage specific DNase are discussed.

MeSH terms

  • Actins / metabolism
  • Adult
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA Primers
  • Deoxyribonuclease I / metabolism
  • Endodeoxyribonucleases / biosynthesis
  • Endodeoxyribonucleases / chemistry
  • Endodeoxyribonucleases / genetics*
  • Female
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Kidney
  • Liver / cytology
  • Liver / enzymology
  • Macrophages / enzymology*
  • Male
  • Mice
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Organ Specificity
  • Polymerase Chain Reaction
  • Pregnancy
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Spleen / cytology
  • Spleen / enzymology
  • Transfection

Substances

  • Actins
  • DNA Primers
  • Oligonucleotide Probes
  • Recombinant Proteins
  • DNASE1L3 protein, human
  • Dnase1l3 protein, mouse
  • Endodeoxyribonucleases
  • Deoxyribonuclease I

Associated data

  • GENBANK/AF047354
  • GENBANK/AF047355